Coding
NosZ

Part:BBa_K4816012:Design

Designed by: Julia Alexander and Matias Brezina   Group: iGEM23_Bonn-Rheinbach   (2023-10-04)


Nitrous Oxide Reductase (NosZ) from Paracoccus denitrificans PD1222


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 600
    Illegal NotI site found at 1937
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1969
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 85
    Illegal NgoMIV site found at 117
    Illegal NgoMIV site found at 160
    Illegal NgoMIV site found at 922
    Illegal NgoMIV site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 34
    Illegal BsaI.rc site found at 1600


Design Notes

Only active sites like nosZ were needed for the project, not the entire enzyme.


Source

Found on KEGG https://www.genome.jp/entry/pde:Pden_4219

References